The Base Excision Repair Pathway Is Required for Efficient Lentivirus Integration

An siRNA screen has identified several proteins throughout the base excision repair (BER) pathway of oxidative DNA damage as important for efficient HIV infection. The proteins identified included early repair factors such as the base damage recognition glycosylases OGG1 and MYH and the late repair factor POLß, implicating the entire BER pathway. Murine cells with deletions of the genes Ogg1, Myh, Neil1 and Polß recapitulate the defect of HIV infection in the absence of BER. Defective infection in the absence of BER proteins was also seen with the lentivirus FIV, but not the gammaretrovirus MMLV. BER proteins do not affect HIV infection through its accessory genes nor the central polypurine tract. HIV reverse transcription and nuclear entry appear unaffected by the absence of BER proteins. However, HIV integration to the host chromosome is reduced in the absence of BER proteins. Pre-integration complexes from BER deficient cell lines show reduced integration activity in vitro. Integration activity is restored by addition of recombinant BER protein POLß. Lentiviral infection and integration efficiency appears to depend on the presence of BER proteins

Physical Fitness Characteristics That Relate to Work Sample Test Battery Performance in Law Enforcement Recruits

This study determined relationships between an agency-specific fitness test battery (PT500), and a work sample test battery (WSTB) in law enforcement recruits. Retrospective analysis on 219 males and 34 females from one agency was conducted. The PT500 comprised: push-ups, sit-ups, and mountain climbers in 120 s; pull-ups; and 201 m and 2.4 km runs. The WSTB comprised: 99 yard (90.53 m) obstacle course (99OC); body drag (BD) with a 165 pound (75 kg) dummy; 6 foot (1.83 m) chain link fence (CLF) and solid wall (SW) climb; and 500 yard (457.2 m) run (500R). Partial correlations, controlling for sex, calculated PT500 and WSTB relationships (p < 0.05). Stepwise regression determined whether fitness predicted WSTB performance. The 500R related to all PT500 assessments (r range = −0.127⁻0.574), 99OC related to all bar push-ups and mountain climbers, and BD related to none. The CLF related to sit-ups, pull-ups, and 2.4 km run; SW related to mountain climbers, pull-ups, and 2.4 km run (r range = −0.127⁻−0.315). Push-ups, pull-ups, and 2.4 km run were involved in predictive relationships for 99OC, CLF, SW, and 500R (r2 range = 0.217⁻0.500). To perform better in the WSTB and job-specific tasks, developing upper-body strength and aerobic fitness may be beneficial